1. Deposit Line & Solvent Front
When preparing your TLC plate, draw the deposit line with a pencil approximately 3 millimeters above the mobile phase level. It’s recommended that the migration height be at least 8 centimeters. Make sure the solvent front migrates no closer than 1 centimeter from the top edge of the TLC plate. 2. How to Make the Deposit
a) Sample ConcentrationTo prevent overloading, it's essential to carefully adjust the concentration. As a general guideline: - For a standard: Use a concentration of 0.1 mg/L. - For samples, the concentration should not exceed 2%.
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b) Deposit SizeSpread the deposit over the smallest possible area to minimize loss of separation. If you plan to make multiple deposits, ensure the plate dries completely between each application. Allow the plate to air dry for about 10 minutes at room temperature before continuing with further development. The size of the deposit significantly affects the separation process. Larger spots tend to spread out more during migration.  |
3. Deposition of the Raw Sample
a) Spot Mode:Using capillarity, either via an HPLC syringe or a capillary, the solute penetrates the adsorbent layer to varying degrees. Deposits made with a syringe tend to be finer compared to those made with a capillary. When using this method, the spots might appear slightly distorted. Load the smallest amount possible to avoid overloading-induced dispersion, which can impact compound resolution. Avoid touching the TLC plate with the depositing tool to reduce excessive penetration of the solute into the plate's layers. Spot density and dispersion may change depending on the solvent polarity. Ensure the spots are placed far enough from the plate's edges to prevent interference.
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b) Spray Mode:With a spray bottle, ensure the spray rate is slower than the solvent's evaporation rate to avoid splashing. This method results in minimal solute penetration into the adsorbent layer, keeping the solutes largely on the surface. This approach yields less dispersed spots, leading to better separation during migration.
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Solvent of DilutionChoose a solvent with low elution power, ensuring it is highly pure. It should be polar enough to dissolve the sample but not excessively so that it evaporates too quickly. Limit the use of bases and acids. Avoid viscous or high boiling point solvents like N,N-Dimethylformamide, DMSO, BuOH, or water, as these can slow down the migration of the solvent. Always allow the plate to dry thoroughly between successive deposits.Solvent of ElutionAdjust the elution strength based on the polarity of the compounds to maintain Rf values within the optimal range (0.15 – 0.35). Note that the mobile phase velocity isn't consistent across the entire plate. Use the same mobile phase for both TLC and flash purification. For a seamless transition to Flash purification: - Measure solvents precisely using separate flasks (double-check the flask's accuracy). - Use a syringe for low-volume measurements to enhance precision. |
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Learn More:
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- Visit our website: www.interchim.com
- Explore our range of purification systems
- Enhance your TLC experience with our "TLC to Flash & Prep Chromatography" app
During the weight loss period, you can drink black coffee, latte, low-fat milk coffee, oatmeal latte, green tea latte and other drinks in moderation.
1. Black coffee
Black coffee does not contain sugar and fat, and will not cause excess calories. Drinking in moderation can refresh the mind and help improve work efficiency. It can also promote gastrointestinal motility, thereby achieving the purpose of assisting weight loss.
2. Latte
Latte is an Italian coffee drink composed of espresso, hot milk and milk foam, usually with a small amount of syrup for flavoring. The caffeine in this drink can increase the metabolic rate, while milk provides certain protein and calcium. Proper intake can help burn fat reserves in the body while maintaining bone health. It is suitable for enjoying before and after exercise or when you need to concentrate.
3. Low-fat milk coffee
Low-fat milk coffee is a drink made by mixing low-fat milk and coffee, in which the saturated fat content in regular whole milk is reduced. This drink retains the slight stimulating effect provided by coffee and reduces the fat content that may cause weight gain. It is suitable as a refreshing drink in the morning or afternoon.
4. Oatmeal Latte
Oatmeal latte is made from oats as the main raw material, rich in dietary fiber, and has a high sense of fullness; coffee contains a certain amount of caffeine, which can stimulate the central nervous system and make people feel excited. Long-term moderate drinking is conducive to promoting gastrointestinal motility, improving the problem of difficult defecation, and thus achieving the effect of weight loss. But be careful not to drink too much, so as not to cause insomnia and other discomfort symptoms.
5. Green tea latte
Green tea latte is a drink made from green tea as raw material. The tea polyphenols contained in it have a certain antioxidant effect; coffee contains a certain amount of caffeine, which can play a diuretic and detumescent effect. Drinking some green tea latte appropriately is good for the body and generally does not affect the weight loss effect. But avoid drinking too much, otherwise you may experience insomnia, palpitations and other symptoms.
It is recommended that when choosing the above foods, pay attention to personal physical differences and possible adverse reactions. For people with high blood pressure, high blood sugar and other diseases, caffeine intake should be reduced to avoid high blood pressure or blood sugar fluctuations.
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