Angiogenesis refers to the differentiation of endothelial cells from an existing vascular network to form new blood vessels. Angiogenesis involves the following processes: migration and proliferation of vascular endothelial cells, degradation of vascular basement membranes, expression of proteolytic enzymes and degradation of extracellular matrices, rearrangement of newly formed extracellular matrices, and formation of endothelial cell vascular walls. The process of angiogenesis involves, under appropriate conditions, endothelial cells can form microvascular or small tubular structures by self-assembly. Anti-angiogenesis has become one of the important strategies for the treatment of diseases such as tumor metastasis, diabetic retinopathy, and rheumatoid arthritis.
As a global leader in cell behavioral analysis, Trevigen offers complete angiogenesis experimental solutions. Among them, the in vitro angiogenesis assay kit and the in vitro angiogenesis endothelial cell infiltration assay reagent are used to evaluate the angiogenic factors at the cellular level, the former can be used to detect endothelial cell tube formation inducers and inhibitors, the latter Accelerate screening of compounds that affect the passage of this vascular endothelial cell through the basement membrane. In addition, Trevigen's patented DIVAA product is the world's first detection system for quantitative detection of angiogenesis in vivo.
Among them, Trevigen's Cultrex® in vitro angiogenesis angiogenesis assay kit detects endothelial cell tube formation inducers and inhibitors. Growth factor-free (RGF)-free BME is a soluble form of basement membrane purified from EHS tumors that solidifies at room temperature to form a layer of fibronectin, type IV collagen, Entactin protein, and heparin sulfate glycoprotein. Reconstitute the protein matrix. The sulforaphane found in broccoli and other cruciferous vegetables is a natural cancer chemical inhibitor that is used as a negative control for inhibitors of endothelial cell formation on Cultrex® BME in vitro. Calcein-AM is used for rapid and accurate detection of cell viability and/or cytotoxicity, as well as real-time kinetic analysis of angiogenesis.
Analysis kit assay kit of Trevigen Cultrex® generating extracorporeal blood vessel formation in vitro angiogenesis contains all the necessary reagents analyzed, including cells for angiogenesis BME matrix components, cell staining agents and fluorescent dyes, and a blood vessel A negative control of the inhibitor formed, and the like. The kit provides an efficient protocol for detecting potential angiogenesis inhibitors in a 96-well format.
| Product name | Brand | Item number | product manual |
| Cultrex® In Vitro Angiogenesis Assay Tube Formation Kit | Trevigen | 3470-096-K | 96 fluorescence analysis |
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