Technical Article Portable UV Analyzer for Detection of DNA Bands

Portable UV analyzer short-wave UV lamp (254nm), portable UV analyzer detection lamp using UV light for fluorescence analysis of substances, has been widely used. The portable UV detection lamp is an ideal small-scale ultraviolet analysis instrument in China. Because of the electronic integrated block starting light source, the instrument is compact and convenient, and it has been widely used by users since its introduction. The LEC-160L portable UV detector lamp can be used to detect DNA-E·B bands in nucleic acid electrophoresis gels and DNA bands in CsC1 density gradient tubes. At the same time, portable UV detection lamps are also used in: biological genetic engineering, molecular genetics, medical hygiene, biological products, pharmaceutical research, health and epidemic prevention, dye chemical, petrochemical, textile industry, public security and political law departments, cultural relics and archaeological departments, where The department for fluorescence analysis can be used.

Fluorescence analysis principle of 254nm short-wave portable ultraviolet analyzer (ultraviolet analyzer) The material molecules in the ground state are absorbed by the excitation light (ie, ultraviolet light), and the excited energy is absorbed, and the material molecules are in an excited state. The energy of the excited state molecule is partially consumed by the vibration, so the molecule is at the vibration level. When the vibration energy level returns to the ground state, the excess energy is emitted in other forms, and the different substances can emit fluorescence of different wavelengths. According to the fluorescence and fluorescence of different light colors, the different substances and contents are judged. This is the fluorescence analysis. This instrument uses a monochromatic light source with a wavelength of 254 nm (short wave). A low-pressure mercury lamp has a strong energy emission at a wavelength of 254 nm. The visible light is filtered through a UV-transparent filter to obtain high-energy ultraviolet monochromatic light for fluorescence analysis.

365nm long wave portable UV analyzer (long-wave UV analyzer) fluorescence analysis principle The analyzer uses low-pressure mercury vapor discharge, low-pressure mercury lamp mainly emits 254nm short-wave ultraviolet light using photon quantum transfer method, coating a characteristic fluorescent material on the inner wall of the lamp tube So that it absorbs 254nm short-wave ultraviolet light and forms a wide ultraviolet spectrum band at 365nm edge, so that it has low power consumption of low-pressure mercury lamp, and it can be used with the filter to remove visible light. High energy long wave UV.

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