How to produce edible fungi cultivar

The strains that are directly used to make edible mushroom cultivation bags or directly sowed are called cultivars, also called production species. With self-expanding cultivars of the original species, the cost of strains can be greatly reduced compared to the direct purchase of cultivars. Raw material selection for the medium. Can use wood chips, cotton seed shell as the main raw material, supplemented with bran or rice bran and so on. The raw materials are required to be fresh, non-toxic and pest-resistant, and wood chips refer to wood chips of hardwood trees such as Cyclobalanopsis glauca and birch. Culture medium preparation. Use wood chips as an example to introduce specific practices. Formula: 78 kg of miscellaneous wood, 20 kg of bran (or rice sugar), 1 kg of sucrose, 1 kg of gypsum powder, material: water = 1:1.2 to 1.5. Before preparation, the bark of the wood block in the sawdust is sieved. Mix the sawdust and bran dry material first, then add the water-sweetened sugar water, mix evenly, and hold the culture medium by hand. When the water between the fingers overflows without dropping, the appropriate water content is 60%. ~ 65%. Bottling. Use 750 ml strain special bottle. Hold the edge and press it tightly to the shoulder. After the surface of the culture medium is flattened, a hole is made in the center of the material with a wooden stick, a layer of plastic film is added to a layer of kraft paper, and the rubber band is used for sealing. Sterilization. Place the filled bottle in the pot. Autoclave steam sterilization, generally 1.2 ~ 1.5 kg / cm2 pressure, continuous sterilization for 1 to 1.5 hours. Inoculation. In the inoculation box, according to the aseptic procedure, pick the thumb of the original species and place it on the center hole of the cultivating medium, and seal it as it is. Mycelial culture. The inoculated seed stock was cultured in a culture chamber at about 25°C. After 2 to 5 days, the strains can germinate the mycelium, and the mycelium resumes growth. Within 5 to 7 days, the hyphae begin to protrude into the medium. In the hypha culture stage, the doors and windows of the culture room are opened every 3 to 4 days for ventilation and ventilation, and each air exchange takes 20 to 25 minutes; the mycelium growth status in the strainer bottle is constantly checked. When edible fungus mycelium grows over the bottle, it is cultivated. China Agricultural Network Editor

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