EM culture fluids are prepared in a clean and sanitized room equipped with screens to prevent flies from entering. Large cylindrical containers, which can be illuminated using electricity, are used to hold over 50 kilograms of water and are sealed with red or white plastic basins. These containers provide an ideal environment for the growth of beneficial microorganisms.
The materials required include 1 kg of EM strain, 1.5–2.5 kg of brown sugar, 92 grams of potassium dihydrogen phosphate, a small amount of honey, and 46 kg of water. If using tap water, it should be left to aerate for more than 48 hours to allow chlorine to dissipate, preventing damage to the cultures.
Before starting, wash the large cylinders and plastic buckets thoroughly. Add honey, potassium dihydrogen phosphate, and brown sugar into the cylinder, then mix with fresh water and shake well. Finally, introduce the EM strain and continue shaking to ensure even distribution. Transfer the mixture into sealed plastic buckets, which will serve as the cultivating containers.
Place the barrels in a sunny area. On cloudy days, supplement with additional lighting in the evening. Arrange four or three barrels in a square or triangular formation, and hang a 60-watt incandescent bulb in the center. Shake the barrels twice daily. If the barrel swells, it indicates high internal pressure due to rapid microbial growth, and the lid should be slightly loosened to release gas.
The optimal incubation temperature is between 22°C and 38°C. Lower temperatures may extend the fermentation time. After 36 hours, stop the light exposure and perform a test: the liquid should appear brown or tan. If it turns milky, the process has failed. The smell should be sweet and sour; if it lacks acidity, the culture may have deteriorated. The pH should be below 3.8. If not, the fermentation may be incomplete or there may not have been enough brown sugar. In such cases, continue fermenting or add more brown sugar and re-ferment until the desired result is achieved.
Once ready, transfer the culture fluid into a mineral water bottle, tighten the cap, and shake it several times. If a significant amount of gas is released, the culture was successful. Some suspended particles are normal and do not indicate failure.
EM culture fluid should not be stored in transparent containers for long periods, as this can cause deterioration. For long-term storage, keep it in a dark, semi-sealed location. The shelf life is approximately one month.
After successful cultivation, the EM fluid can be used to produce fly larvae. One method involves mixing 80% pig manure with 16% wheat bran and 4% EM live bacteria. Alternatively, use 56% pig manure, 40% chicken manure, and 4% EM culture fluid.
Mix the above fecal material with the EM culture solution—add 5 kg of culture per ton of material—to achieve a moisture content of 90%. Cover with a film and ferment under sunlight. Turn the pile on the third day, then add another 3 kg of EM culture solution to adjust the pH to between 6.5 and 7. If the pH is above 7, add vinegar; if below 6.5, add lime water. Once ready, move the mixture to the nursery room and form it into rows. Place some dead fish to attract flies for egg-laying. Other breeding techniques follow standard procedures.
Waterproof Wound Dressing,Rectangle Big Wound Dressing,Waterproof Adhesive Wound Dressing,Medical Fixation Wound Dressing,Sterile Wound Dressing,IV Cannula Dressing
Surgimed Medical Supplies Co.,Ltd , https://www.surgimedcn.com