Operation method and precautions of inoculation ring sterilizer in microbial experiment

In the microbiology laboratory, many experimenters habitually discard the used medium (containing bacteria) directly, without thorough sterilization, which not only pollutes the safety of the laboratory itself, but also causes great Environmental pollution, the harm is very large, in the process of SARS virus research, the laboratory has been infected by the test researchers, which is very dangerous and not worth the loss. Since traditional alcohol lamps cannot be used in biosafety cabinets, although this relatively inexpensive alcohol is used for a long time as a sterilization device that we often use for microbial sterilization, it is increasingly unable to satisfy us to a certain extent. The requirements of the experiment, the production of the inoculation ring sterilizer largely solved these problems. This kind of no fire, easy to operate, and the sterilization time only takes 5-6 seconds to greatly save the experiment time. But in use We are also often prone to many primary problems:

Be careful when using an inoculation ring sterilizer. I often use it when I do experiments, and sometimes I am a little impatient when I do more. Especially when sterilizing the coarse inoculation ring, it is anxious to squat on it. If you don’t burn it, you can hold it anyway. Do something else first, such as writing a number on the test tube, and writing it. We use the inoculation ring again, and it is just enough to use it. Generally, it's okay, but it's easy to have a set of experiments to make the last one, because the vaccination ring is no longer used. It is often thought that when things are cleaned up, it is a terrible thing to look at the vaccination ring. Seriously changed shape. Therefore, we remind us that the experimenter must not easily and completely destroy the whole experiment when doing the inoculation experiment.

If you find that the inoculation ring plastic bar is slightly inflated where it meets the metal bar, or if the plastic bar is slightly bent down, then don't worry, it will save. Turn off the sterilizer power supply, take a tweezer in one hand, and clamp the metal rod and plastic rod of the inoculating ring separately. Carefully remove it from the sterilizer and lay it on the test bench. (Do not touch the paper or gauze, it will catch fire. If the test bench is not heat-resistant, you need to put a metal plate on it, so that the plastic rod and the metal rod are in a straight line. After waiting for it to cool, you can use it again, but the expanded place will not be retracted. You leave a "memorial".

If you find that the inoculating ring has been burned into a "drawing iron bar", or the plastic bar has been burnt into "sweet", then you have to move faster, otherwise "black syrup" will stick everywhere. At this time, turn off the sterilizer power supply, take a waste liquid tank and hold a large half of the water. Use two tweezers to hold the complete part of the plastic rod and the metal rod (do not clip the “sweet” or “drawing” parts. And it will stick to the scorpion), carefully remove it, put it in the water to cool it, remove the wire that can be reused, and then throw it away. If the waste cylinder is plastic, be careful not to let the metal rod touch the waste tank, otherwise they will not be separated after "intimate contact". If you are close to the pool, you can also use tap water to cool. Never put the inoculum ring directly on the table, so that the melted glue will stick to the table, and you will scrape it slowly.

In either case, you can't take the metal rod or the plastic rod directly by hand. Unless you make up your mind, you must leave a "mark" on your hand to make your heart "small".

Don't leave the inoculum in the sterilizer and let it cool itself, because there is still heat left after the sterilizer is turned off, leaving the loop in it will only make things worse.

Cardiovascular & Urinary

Cardiovascular:

Relating to the circulatory system, which comprises the heart and blood vessels and carries nutrients and oxygen to the tissues of the body and removes carbon dioxide and other wastes from them. Cardiovascular diseases are conditions that affect the heart and blood vessels and include arteriosclerosis, coronary artery disease, heart valve disease, arrhythmia, heart failure, hypertension, orthostatic hypotension, shock, endocarditis, diseases of the aorta and its branches, disorders of the peripheral vascular system, and congenital heart disease.Related Products:cardiovascular drugs.

Urinary:

For the treatment ofl some diseases of the urinary system.

Different specialists treat urinary system ailments. Nephrologists treat kidney diseases, while urologists treat problems with the urinary tract, including the kidneys, adrenal glands, ureters, bladder and urethra, according to the American Urological Association (AUA). Urologists also treat the male reproductive organs, while gynecologists often treat urinary diseases or disorders in females, including yeast infections. Nephrologists and urologists often work with endocrinologists or oncologists, depending on the disease.

Urinary tract infections (UTIs) occur when bacteria enters the urinary tract and can affect the urethra, bladder or even the kidneys. While UTIs are more common in women, they can occur in men. UTIs are typically treated with antibiotics, according to Dr. Oscar Aguirre, a urogynecologist in Denver.

Incontinence is another common disease of the urinary system. It can come in the form of a pelvic prolapse, which can result in leakage and can be the result of a vaginal

delivery. Then there is the overactive bladder, [which we see a lot and is not related to having children or trauma," Aguirre said. A third condition involves overflow, in which the bladder does not completely empty.

Some common treatments involve medications, physical therapy and pelvic mesh surgery, Aguirre noted. Vaginal laser surgery is also becoming a viable treatment option, he explained. [In another 10 to 15 years, vaginal laser surgery will be another common option for the treatment of urinary conditions."

Interstitial cystitis (IC), also called painful bladder syndrome, is a chronic bladder condition, primarily in women, that causes bladder pressure and pain and, sometimes, pelvic pain to varying degrees, according to the Mayo Clinic. It can cause bladder scarring, and can make the bladder less elastic. While the cause isn`t known, many people with the condition also have a defect in their epithelium, the protective lining of the bladder.

Prostatitis is a swelling of the prostate gland and, therefore, can only occur in men. Often caused by advanced age, symptoms include urinary urgency and frequency, pelvic pain and pain during urination, the Mayo Clinic noted.

Kidney stones are clumps of calcium oxalate that can be found anywhere in the urinary tract. Kidney stones form when chemicals in the urine become concentrated enough to form a solid mass, according to the Cleveland Clinic. They can cause pain in the back and sides, as well as blood in the urine. Many kidney stones can be treated with minimally invasive therapy, such as extracorporeal shock wave lithotripsy, which disintegrates the kidney stones with shock waves.

Kidney failure, also called renal failure and chronic kidney disease, can be a temporary (often acute) condition or can become a chronic condition resulting in the inability of the kidneys to filter waste from the blood. Other conditions, such as diabetes and hypertension, can cause chronic kidney disease, according to the Mayo Clinic. Acute cases may be caused by trauma or other damage, and may improve over time with treatment. However, renal disease may lead to chronic kidney failure, which may require dialysis treatments or even a kidney transplant.

Bladder cancer is diagnosed in about 75,000 Americans each year and is more frequent in men and the elderly according to the American Cancer Society. The symptoms, including back or pelvic pain, difficulty urinating and urgent/and or frequent urination, mimic other diseases or disorders of the urinary system.

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