Application and Prospect of Liquid Flash Measurement Technology in Traditional Chinese Medicine Research

1. The liquid flash measurement of eucalyptus is a technique for the in vitro radioactivity measurement using liquid scintillation counting 嚣. It is most beneficial to detect the soft beta rays of the penetrating power of ³ H, ¹⁴ C, etc. emitted from the isolated samples. It has the advantages of high sensitivity and strong specificity. The application of this technology has made the research of traditional Chinese medicine deeper, and has become a channel for communication between traditional medicine and modern medicine, and has made great contributions to the study of the combination of Chinese medicine and medicine. So far, the technology has been applied to various fields of traditional Chinese medicine research. The relevant information is summarized below:

1 Application in Chinese herbal medicine research

Radioimmunoassay accounts for more than 70% of the established methods for the detection of active ingredients in Chinese herbal medicines. Because of the H mark. Therefore, liquid flash measurement technology is particularly important. For example, the establishment of artemisinin artemisia annua test method has been applied by foreign scholars to establish a method for the determination of total VD derivatives in calcareous plants. Waiting for the rich resources of Chinese herbal medicines in China, it is an arduous task for Chinese medicine research to find new active ingredients.

In recent years, liquid flash measurement technology has been widely used in pharmacology research of traditional Chinese medicine. It can not only be used to determine the crude drug and its dare ingredients in the human body, but also the changes in the content of various biological active substances in the human body after administration, and it has made great contributions to the pharmacological research of Chinese medicine to the molecular level.

In the study of the active ingredients of traditional Chinese medicine, the content of cyclohexaic acid in traditional Chinese medicines such as Taizao, Yinzhi and Salvia, and the testosterone, progesterone, estradiol and other steroid hormones in the scorpion oil can be measured by liquid flash. Zhou Wen Bath et al. ³ H Berberine ³ H. Bracts are tracers. Observing the transdermal absorption of external application of traditional Chinese medicine anti-inflammatory, using the liquid idle counter to detect the blood-staining tissue, feces and urine of the rats used in the test, the results showed that: ³ H borneol may not be enough transdermal volume 5% of the amount, the external application of ³ H small base is also mostly left in vitro; suggesting that external application of Chinese medicine can partially penetrate the skin, but the amount of transdermal is limited. And it varies with components. It is speculated that the topical Chinese medicine may play a part of direct action on the diseased tissue, and may also act through other mechanisms.

According to the principle of radioimmunity. 4. The use of liquid helium measurement for the determination of thromboxane in vitro samples. The content of trace substances such as prostaglandins, cyclic adenosine monophosphate, and ketone ketone. Observing the changes in the content of these trace substances after administration is an important basis for elucidating the mechanism of action of traditional Chinese medicines and guiding clinical medication. Deng Changqing et al. tested the effects of TX&6-keto prostaglandin and plasma and felt ring phosphate on cerebral ischemia-reperfusion injury model in rabbits. The mechanism of action of Yiqi Huoxue Recipe on Astragalus and Huoxue Recipe was compared. It was found that Huoxue Decoction and Yiqi Huoxue Recipe can inhibit the increase of brain TX after re-injection of rabbit brain and blood, and increase the content of 6-keto-prostaglandin R in brain. Huoxue Recipe can also inhibit the elevation of brain cAMP, and jaundice has no significant effect on this. It is suggested that the Yiqi Huoxue method works by improving the local TXA2/PGI: balance in the brain, and the effect of the blood-activating drug is mainly detected in the study of the hippocampus and the hypothalamic-coagulation-adrenal axis (HPA axis) of the aged rats. Plasma corticosterone content, found that Guzheng can significantly reduce the increased plasma cortisol levels in aged rats, the results of the analysis of glucocorticoid receptors, deepen the understanding of the effect of phlegm on delaying hippocampus and HPA axis degeneration in aged rats. In the study of the mechanism of lysis and antipyretic effects, Yu Linzhong found that the drug can inhibit the synthesis and release of PGB and cAMP in the central febrile medium, and then affect the hypothalamic temperature regulation center.

In recent years, combined with molecular biology techniques, liquid scintillation measurement technology has played a significant role in the process of pharmacological research of traditional Chinese medicines reaching the molecular level. Lee should Dong et al in peripheral blood lymphocytes roar unscheduled DNA units into (the UDS) were observed after, synthetic precursor material with ³ H labeled DNA, by liquid scintillation Measurement of genotoxicity teaching Curcuma wood and nickel sulfate should Ha Curcuma of The effect of nickel sulfate on the peripheral blood lymphocytes UDS. It was found that sputum can significantly reduce the DNA damage of peripheral blood lymphocytes of nickel sulfate at a certain dose, but it has no mutagenic effect. However, when the dose of sputum is 1g/ml, the repair of DNA has been inhibited. In addition, the author I used the ³ H methyl incorporation method to determine the DNA methylase activity in rat brain in the anti-aging Chinese medicine original study. It was found that the specific activity of the enzyme increased after long-term use of Shenfang Bujiaosheng. The thermal stability of the enzyme and the tolerance to NaC [slightly increased. And the folding of the protein component of the protein indicates that the blood-methylase activity and enzymatic properties of the brain of the rats can be changed. Thus affecting the level of DNA methylation may be one of the ways of anti-aging effects of the drug. The authors also used H-incorporation method to detect DNA polymerase and reverse transcriptase activity, which confirmed that Shugan Lidan Granule has obvious inhibitory effect on the above two enzymes, and provides experimental basis for clinical drug use.

2 Application of basic theoretical research in Chinese medicine

The basic theory of traditional Chinese medicine has rich connotations, but the current Chinese medicine still does not have the ability to summarize the basic characteristics of the basic theory. It uses modern detection technology in the process of transforming from empirical medicine to scientific medicine. Necessary and respectful. In the experimental study of the principle of tongue coating, it was found that the Ac MP and cGMP of the thick moss increased simultaneously, suggesting that the cell division and differentiation accelerated, while the keratinization and shedding were relatively delayed; the pathological white Bodhisattva was lower than normal, and the thin yellow moss was two. All were higher than normal, suggesting that the division and differentiation are at different levels of relative balance; the higher levels of cAMP and eGMP in exfoliants may be related to the premature degeneration and necrosis of some maternal cells.

Qian Yanhong et al. In the experimental study of internal dryness and internal wetness, five groups of rats (constant body cold, phlegm, internal dryness group, internal wet group) were studied separately, and their peripheral blood lymphocyte DNA was analyzed. Ultraviolet radiation damage was measured by H incorporation and collection of cell liquid. By observing the difference in repair ability of lymphocytes after DNA damage, the cytological basis of the type of constitution and its transformation was further explored. The results showed that the ³ H TdR intrusion value of the internal dry group was lower than that of the hot body group, and the ³ H -TdR incorporation value of the internal wet group was smaller than that of the cold group, and the difference was significant, suggesting that the ³ H TdR incorporation value of DNA can be used as the difference. One of the indicators of normal constitution and pathological constitution.

In the acupuncture study, the application of liquid idle measurement of acupuncture, the changes in the amount of trace minerals such as glucosinolate, thrombus, prostacyclin and so on in the body beach and tissue also contribute to the study of the mechanism of acupuncture. Research has shown that. Acupuncture at Neiguan can inhibit excessive elevation of cAMP in ischemic cardiomyocytes, prevent arrhythmia caused by increased cd permeability of the cell membrane, reduce myocardial oxygen consumption, and reduce Ac MP and cGMP content in the medulla oblongata. The medullary cardiovascular center is improved to improve the cardiac function status of patients with acute myocardial hemorrhage; the volume of cAMP in the blood is reduced, indicating that the internal acupuncture can inhibit the excessive strong stress response of the body. The heart of the port is exacerbated. Good for the recovery of ischemic myocardium. Other studies have shown that "acupuncture heart meridians and needles touch the lungs through the meridians have antihypertensive effect, and the intensity of decompression of acupuncture and moxibustion is greater than that of acupuncture lung. The increase of serum prostacyclin level is the mechanism of acupuncture hypotension. one.

3 Chinese medicine clinical research applications

The clinical practice of traditional Chinese medicine is based on syndrome differentiation. According to the data obtained from the patients, the doctors are asked to read, smell, ask, and cut, and the eight-class, dirty and phlegm dialectical comprehensive Nadan type, and according to different types of treatment should be treated, the application of liquid flash measurement technology Correlate detection of TCM disease classification and scientific analysis of disease etiology, pathological outcome and prognosis. It not only provides a scientific basis for clinical syndrome differentiation and treatment of traditional Chinese medicine, but also clarifies the link between the quality of the Chinese medicine and the role of the drug.

The liquid idle measurement technique was used to detect the active substances or other functional indicators in the body of TCM syndrome patients, and the relationship between them and TCM classification was observed based on the basis of syndrome differentiation. For example, the clinical observation of urinary PGE2 and PGF2 excretion in patients with deficient cold and deficiency of heat syndrome found that the urinary PGE2 output decreased in patients with deficient cold: PGF2 was not significantly changed in urinary PGE2 output. The PGE/PGF2 value is elevated. It is suggested that PGE2 and PGF2 are closely related to TCM syndrome differentiation.

4 prospects and prospects

In recent years, researchers have done a lot of practical work in the research of traditional Chinese medicine by using liquid flash volume technology, and have already explored the fields of science, law, prescription and medicine of Chinese medicine, providing science for clinical syndrome differentiation of traditional Chinese medicine. According to the research, it has obtained valuable data for further in-depth research. Although liquid flash measurement technology has the disadvantages of difficult treatment of radioisotope waste and expensive reagents, it is fast, accurate and specific, and the sample does not need to be purified. The outstanding advantages of trace amounts (minimum measured values ​​up to 10 ^-15 levels) are still not replaced by other methods. and. This technology is combined with other related technologies such as molecular biology. Make Chinese medicine research more interesting. It even penetrates into topics that could not be studied in the past.

In summary. Actively using modern science and technology to promote the development of traditional Chinese medicine has become the consensus of most contemporary Chinese medicine practitioners. Liquid flash measurement technology is playing an increasingly important role in the research of traditional Chinese medicine. I believe that with the deepening and development of research. It will certainly make greater contributions to the discovery of Chinese medicine and the modernization of Chinese medicine.
( Tianjin College of Traditional Chinese Medicine Wang Hong )