The disease is caused by the poisoning of the sheep eating the roots of Valerian plants. It is characterized by blindness in the eyes and dilated pupils, followed by general spasm and bladder paralysis, and accumulation of urine. It is known as blind eye disease.
Experts solve the disease. (1) Etiology: Alfalfa root is also known as day lily root and day lily flower root. Alfalfa root poisoning is caused by the poisoning caused by the sheep feeding on alfalfa roots. The disease mostly occurred during the 2-3 months of the transplanting and renewal of valerian roots. The roots of valerian roots were plucked out of the ground and mostly abandoned in the wild. Due to the dry feeding period, the grazing sheep once rushed to eat the fresh grassroots, resulting in a large number of sheep. Poisoning died.
(2) Symptoms: The speed and severity of the symptoms of the diseased sheep depends on the amount of food consumed by the sheep. In the early stages of the disease, the diseased hens suffered from reduced or absent appetite, sluggishness, and urine was orange-red. Then the mouth bleeds, the pupils gradually diverge, and the two eyes are blind or one after the other. The sick sheep panic and mourn. The aimless chase or abut obstacles. After falling to the ground, the limbs keep moving, like swimming. Some muscles in the extremities twitched and walked weakly, especially in the rear limbs. They eventually paralyzed and could not afford lying. In the late period, the jaws are tightly closed, chewing is difficult, sometimes the teeth are gritted, breathing is difficult, and the heart beats faster, usually dying after 2-4 days. Poorly poisoned ones can recover, but blind eyes with dilated eyes cannot recover.
Expert treatment. 1 Prevention: Forage season prohibits sheep from grazing on daylilies with daylily, and properly keeps and handles discarded or transplanted daylily.
2 treatment: There is no specific detoxification method. Grazing should stop grazing after the onset of the disease. Early in the process, salt laxatives can be administered, high-quality hay and fodder can be given, nursing care can be strengthened, and antibiotics can be used to prevent secondary infection. At the same time intravenous glucose saline can help the recovery of the disease.
The enzyme-linked immunosorbent assay (ELISA) (/ɪˈlaɪzÉ™/, /ËŒiËˈlaɪzÉ™/) is a commonly used analytical biochemistry assay, first described by Engvall and Perlmann in 1971.[1] The assay uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries.
In the most simple form of an ELISA, antigens from the sample are attached to a surface. Then, a matching antibody is applied over the surface so it can bind to the antigen. This antibody is linked to an enzyme, and in the final step, a substance containing the enzyme's substrate is added. The subsequent reaction produces a detectable signal, most commonly a color change.
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