First, the cultivation time
The inoculation period for the back-season cultivation is from June to July. After 20 to 30 days, the fungus grows. From July to August, the summer passes, and from the end of September to October, the mushroom grows.
Second, culture material formula
Formula One: 94% cotton shell, 2.8% corn flour, 1% plaster, 2% right ash, 0.2% urea. Formulation 2: 40% cotton seed hull, 40% wood chips, 10% bran, 8% corn flour, 1% sugar, and 1% plaster. The above formulation moisture content is maintained at 60% to 65%.
Third, the preparation of culture materials and fermentation
The water-soluble sugars, urea, etc. are dissolved in water, and the rest of the raw materials are mixed and mixed with water and mixed repeatedly, and piled into a trapezoidal pile having a width of 1 to 1.5 meters, a height of 1.2 to 1.5 meters, and an unlimited length. Each pile of 30 to 50 cm on the pile to play a breathable hole, its aperture is 7 to 10 cm, each side play two rows of vents. The top cover membrane is moisturized, and the bottom of the stack is 30 to 50 cm without covering the membrane. When the temperature of the reactor rises to 65°C to 70°C, the reactor is turned and the fermentation is carried out for 7 to 10 days, turning the reactor 3 times.
Fourth, bagging
Bailing mushrooms are used in bags. The cultivation bag is made of a high-density polyethylene or polypropylene film with a thickness of 0.04 to 0.06 cm. The width is 15 to 17 cm and the length is 35 cm. Before the bagging, the fermented culture medium was adjusted to have a water content of 60% and a pH of 7.5-8.5. When bagging, one end of the bag is tied well, and the culture material is evenly packed into the bag. Each bag contains 0.95-1.05 kg of culture material.
Fifth, sterilization
The filled bags are autoclaved for 2 to 3 hours and sterilized at normal pressure for 10 to 18 hours. After sterilization, remove the bag and cool it in the inoculation room or in a clean place.
VI. Inoculation
When the bag temperature dropped to 30°C, the bag opening was opened in the inoculation room or inoculation box, and the bacteria species were inserted from both ends or one end under aseptic conditions, and the inoculation amount was 10% of the culture material.
Seven, bacteria management
The species of bacteria bag was taken into the culture room and stacked on the bed frame. The culturing chamber was sprayed with carbendazim for disinfection before being placed in the bacterial bag. After being placed in the bacterium bag, it was sprayed and disinfected once every 5 to 7 days. The temperature of the culturing chamber is controlled at 22°C to 27°C, and the air humidity is maintained at about 70%. Avoid light culture. Germ fungus 10 to 15 days, when the mycelium at both ends of the material surface, the turning. Breeding 35 to 40 days mycelium can send a full bag.
Eight, summer bags
After the bacteria bag is placed in the over-summer place, the humidity should be checked regularly and ventilated. When the temperature in the fall cools, the bacteria bags will be moved into the mushroom house for management.
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