Receptor egg cytoplasm controls and influences the development and morphogenesis of cloned fish to a considerable extent. The research team led by Zhu Zuoyan, a member of the Chinese Academy of Sciences and a researcher at the Institute of Hydrobiology, Chinese Academy of Sciences, discovered for the first time the cytoplasmic effect of cloned fish on the molecular level. New evidence. According to an article published in the latest issue of "Reproductive Biology" published this month by academician Zhu Zuoyan and others, "The Effect of Cytoplasm on the Development of Intergeneric Clone Fish Derived from the Nucleus of a Transgenic Carp and the Goldfish Enucleation Egg": Report by Zhu Zuoyan The research team used the embryonic nuclei of the growth hormone gene carp as a donor and the goldfish to nucleate unfertilized eggs as receptors for intergeneric cloning. In a total of 501 operational eggs, seven genus normally developed as adult fish were obtained. Clone the fish. The shape of the cloned fish resembles that of the nucleus donor carp rather than that of the recipient goldfish. The PCR detection of the transgene and the RAPD analysis of the total DNA confirm that the nuclear genomic DNA of the cloned fish is derived from the donor carp; but the donor carp is A follow-up study of mitochondrial DNA of recipient goldfish in cloned embryos and cloned fish found that cloned embryos before the circulation period coexisted both mitochondrial DNA derived from both donor and acceptor types, and cloned embryos at subsequent developmental stages. Up to the cloned fish individuals, only mitochondrial DNA of the recipient cytoplasm was present; in 7 cloned fish, the number of vertebrae of 6 individuals was 26-28, the number of vertebrae of 1 individual was 31, and the number of vertebrae that were usually spasmodic It is 33 to 36, and the number of vertebrae of goldfish is 26 to 28. The experimental data show that the receptor egg cytoplasm plays a very important role in the development and trait determination of cloned fish. For the first time in this study, the discovery of new evidence of cytoplasmic effects on the development of cloned fish from the molecular level has important scientific and social significance.
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